Research Summary:

Peter Lansdorp is a pioneer in studies of blood-forming stem cells, telomeres and the biology of ageing with a track record of developing novel ideas and approaches to address diverse scientific questions. As such, Peter Lansdorp has made contributions to multiple, apparently distinct research fields. He has published over 300 papers, a hundred of which have been cited more than a 100 times (total citations > 40,000; h-index=108). From 2011-2016 he was the Founding Director of the European Research Institute for the Biology of Ageing (ERIBA) at the University Medical Center Groningen and the University of Groningen in the Netherlands. He returned to UBC in 2017.
As a graduate student Peter Lansdorp discovered tetrameric antibody complexes. This discovery has found numerous applications including cell separation techniques (commercialized by StemCell in Vancouver). For his research on human hematopoietic stem cells he developed assays, monoclonal antibodies (such as anti-CD34), cell culture media and cell separation techniques that are still in widespread use. In 1994 his lab found that the length of telomere repeats in hematopoietic cells decreases with proliferation in vitro and with age in vivo. He subsequently developed quantitative fluorescence in situ hybridization (Q-FISH) techniques, using directly labeled peptide nucleic acid (PNA) probes, to measure the length of telomere repeats in chromosomes and cells. Most laboratories involved in telomere research use PNA probes according to the methods developed by the Lansdorp laboratory. In 2005 he founded a Vancouver company (Repeat Diagnostics Inc.) that provides telomere length measurements as a diagnostic service to clinicians worldwide looking after patients with aplastic anemia, idiopathic pulmonary fibrosis and other “telomere” disorders. A current interest in his laboratory is related to the possibility that gene expression and cell fate are regulated in part by chromatin differences between sister chromatids. In order to test the “silent sister” hypothesis his laboratory developed a single cell DNA strand sequencing method. The Strand-seq method has widespread applications in studies of genetics, aging and cancer.

MD Erasmus University, Rotterdam, The Netherlands Internal Medicine
PhD University of Amsterdam, The Netherlands Experimental Hematology
Recent Publications:
  • van Wietmarschen N*, Merzouk S*, Halsema N*, Spierings DCJ*, Guryev V, Lansdorp PM. (2018). BLM helicase suppresses recombination at G-quadruplex motifs in transcribed genes. Nat. Commun. 9:271-278.
  • Lansdorp P.M. (2017). Maintenance of telomere length in AML. Blood Advances. 25: 2467-2472.
  • Claussin C, Porubský D*, Spierings DC*, Halsema N*, Rentas S, Guryev V, Lansdorp PM, Chang M. (2017). Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq. Elife. 6: pii:e30560.
  • O’Neill K, Hills M*, Gottlieb M, Borkowski M, Karsan A & Lansdorp PM. (2017). Assembling draft genomes using contiBAIT. Bioinformatics. 33: 2737.
  • Levine MS, Bakker B, Boeckx B, Moyett J, Lu J, Vitre B, Spierings DC*, Lansdorp PM, Cleveland DW, Lambrechts D, Foijer F, Holland AJ. (2017). Centrosome amplification is sufficient to promote spontaneous tumorigenesis in mammals. Dev Cell. 40: 313-322.
  • Porubsky D*, Garg S, Sanders AD*, Korbel JO, Guryev V, Lansdorp PM, Marschall T. (2017). Dense and accurate whole-chromosome haplotyping of individual genomes. Nat.Commun.8: 1293.
  • Lansdorp, P.M. (2017). Maintenance of telomere length in AML. Blood Advances. 25: 2467-2474.
  • Ferronika P, van den Bos H*, Taudt A*, Spierings DCJ*, Saber A, Hiltermann TJN, Kok K, Porubsky D*, van der Wekken AJ, Timens W, Foijer F, Colomé-Tatché M, Groen HJM, Lansdorp PM, van den Berg A. (2017). Copy number alterations assessed at the single-cell level revealed mono- and polyclonal seeding patterns of distant metastasis in a small cell lung cancer patient. Ann.Oncol.28: 1668.
  • Foijer F, Albacker LA, Bakker B, Spierings DC*, Yue Y, Xie SZ, Davis SH, Lutum-Jehle A, Takemoto D, Hare B, Furey B, Bronson RT, Lansdorp PM, Bradley A & Sorger PK. (2017). Deletion of the MAD2L1 spindle assembly checkpoint gene is tolerated in mouse models of acute T-cell lymphoma and hepatocellular carcinoma. Elife. 6: pii: e20873.
  • Soto M, Raaijmakers JA, Bakker B, Spierings DCJ*, Lansdorp PM, Foijer F & Medema RH. (2017). p53 prohibits propagation of chromosome segregation errors that produce structural aneuploidies. Cell Rep. 19:2423-2431.
  • Kazemier HG, Paeschke K & Lansdorp PM. (2017). Guanine quadruplex monoclonal antibody 1H6 crossreacts with restrained thymidine-rich single stranded DNA. Nucleic Acids Res. 45: 5913.
  • van den Bos H*, Spierings DC*, Taudt AS*, Bakker B, Porubský D*, Falconer E*, Novoa C*, Halsema N, Kazemier HG, Hoekstra-Wakker K, Guryev V, den Dunnen WF, Foijer F, Tatché MC, Boddeke HW, & Lansdorp PM. (2016). Single-cell whole genome sequencing reveals no evidence for common aneuploidy in normal and Alzheimer’s disease neurons. Genome Biology. 17: 116.
  • van Wietmarschen N* & Lansdorp PM. (2016). Bromodeoxyuridine does not contribute to sister chromatid exchange events in normal or Bloom syndrome cells. Nucleic Acids Research. 44: 6787-93.
  • Lansdorp PM. (2016). Telomeres on steroids: turning back the mitotic clock? New England Journal of Medicine. 374: 1978-20.
  • Porubsky D*, Sanders AD*, van Wietmarschen N*, Falconer E*, Hills M*, Spierings*, D. Bevova MR*, Guryev V, Lansdorp PM. (2016). Direct chromosome-length haplotyping using single cell DNA template strand sequencing. Genome Research. 26(11): 1565-1574.
  • Sanders AD*, Hills M*, Porubsky D*, Guryev V, Falconer E*, Lansdorp PM. (2016). Characterizing polymorphic inversions in human genomes by single cell sequencing. Genome Research. 26(11): 1575-1587.
  • Bakker B, Taudt A*, Belderbos ME, Porubsky D*, Spierings DC*, de Jong TV, Halsema N, Kazemier HG, Hoekstra-Wakker K, Bradley A, de Bont ES, van den Berg A, Guryev V, Lansdorp PM, Colomé-Tatché M, Foijer F. (2016). Single-cell sequencing reveals karyotype heterogeneity in murine and human malignancies. Genome Biology. 17: 115.
  • Bakker B, van den Bos H*, Lansdorp PM, Foijer F. (2015). How to count chromosomes in a cell: An overview of current and novel technologies. Bioessays. 37: 570-7.
  • Benchetrit H, Herman S, van Wietmarschen N*, Wu T, Makedonski K, Maoz N, Yom Tov N, Stave D, Lasry R, Zayat V, Xiao A, Lansdorp PM, Sebban S, Buganim Y. (2015). Extensive nuclear reprogramming underlies lineage conversion into functional trophoblast stem-like cells. Cell Stem Cell. 17: 543-56.
  • Buganim Y, Markoulaki S, van Wietmarschen N*, Hoke H, Wu T, Ganz K, Akhtar-Zaidi B, He Y, Abraham BJ, Porubsky D, Kulenkampff E, Faddah DA, Shi L, Gao Q, Sarkar S, Cohen M, Goldmann J, Nery JR, Schultz MD, Ecker JR, Xiao A, Young RA, Lansdorp PM, Jaenisch R. (2014). The developmental potential of iPSCs is greatly influenced by reprogramming factor selection. Cell Stem Cell. 15: 295-309.
  • Gardner M, Bann D, Wiley L, Cooper R, Hardy R, Nitsch D, Martin-Ruiz C, Shiels P, Sayer AA, Barbieri M, Bekaert S, Bischoff C, Brooks-Wilson A, Chen W11, Cooper C, Christensen K, De Meyer T, Deary I, Der G, Diez Roux A, Fitzpatrick A, Hajat A, Halaschek-Wiener J, Harris S, Hunt SC, Jagger C, Jeon HS, Kaplan R, Kimura M, Lansdorp P, Li C, Maeda T, Mangino M, Nawrot TS, Nilsson P, Nordfjall K, Paolisso G, Ren F, Riabowol K, Robertson T, Roos G, Staessen JA, Spector T, Tang N, Unryn B, van der Harst P, Woo J, Xing C, Yadegarfar ME, Park JY, Young N, Kuh D, von Zglinicki T, Ben-Shlomo Y; Halcyon study team. (2014). Gender and telomere length: systematic review and meta-analysis. Experimental Gerontology. 51: 15-27.
  • Lisaingo K*, Uringa EJ*, Lansdorp PM. (2014). Resolution of telomere associations by TRF1 cleavage in mouse embryonic stem cells. Mol Biol Cell. 25: 1958-68.
  • Chiang YJ, Calado RT, Hathcock KS, Lansdorp PM, Young NS & Hodes RJ. Telomere length is inherited with resetting of the telomere set point. Proc Natl Acad Sci USA 107: 10148-10153, 2010.
  • Falconer E, Chavez E, Henderson A & Lansdorp PM. Chromosome orientation fluorescence in situ hybridization to study sister chromatid segregation in vivo. Nat Protoc 5: 1362-1377, 2010.
  • Falconer E, Chavez EA, Henderson A, Poon SSS, McKinney S, Brown L, Huntsman DG & Lansdorp PM. Identification of sister chromatids by DNA template strand sequences. Nature 463: 93-7, 2010.
  • Gourronc FA, Robertson MM, Herrig AK, Lansdorp PM, Goldman FD & Klingelhutz AJ. Proliferative defects in dyskeratosis congenita skin keratinocytes are corrected by expression of the telomerase reverse transcriptase, TERT, or by activation of endogenous telomerase through expression of papillomavirus E6/E7 or the telomerase RNA component, TERC.  Exp Dermatol  Volume 19, Issue 3, Date: March 2010, Pages: 279-288.
  • Kimura M, Gazitt Y, Cao X, Zhao X, Lansdorp PM & Aviv A. Synchrony of telomere length among hematopoietic cells. Exp Hematol 38: 854-859, 2010.
  • Banath JP, Banuelos CA, Klokov D, MacPhail SM, Lansdorp PM & Olive PL. Explanation for excessive DNA single-strand breaks and endogenous repair foci in pluripotent mouse embryonic stem cells.  Exp Cell Res 315: 1505-1520, 2009.
  • Breakey VR, Meyn S, Ng V, Allen C, Dokal I, Lansdorp PM, Abla O & Dror Y. Hepatitis-associated aplastic anemia presenting as a familial bone marrow failure syndrome.  J Pediatr Hematol Oncol 31: 884-887, 2009.
  • Calado RT, Regal JA, Hills M, Yewdell WT, Dalmazzo LF, Zago MA, Lansdorp PM, Hogge D, Chanock SJ, Estey EH, Falcao RP & Young NS. Constitutional hypomorphic telomerase mutations in patients with acute myeloid leukemia.  Proc Natl Acad Sci USA 106: 1187-1192, 2009.
  • Calado RT, Regal JA, Kleiner DE, Schrump DS, Peterson NR, Pons V, Chanock SJ, Lansdorp PM & Young NS. A spectrum of severe liver and blood disorders associate with telomerase mutations.  PLoS One 4: e7926 2009.
  • Decker ML, Chavez E, Vulto I & Lansdorp PM. Telomere length in Hutchinson-Gilford progeria syndrome.  Mech Ageing Dev 130: 377-383, 2009.
  • Falconer E, Chavez EA, Henderson A, Poon SSS, McKinney S, Brown L, Huntsman DG & Lansdorp PM. Identification of sister chromatids by DNA template strand sequences.  Nature 463: 93-7, 2009.
  • Hills M & Lansdorp PM. Short telomeres resulting from heritable mutations in the telomerase reverse transcriptase gene predispose for a variety of malignancies.  Ann N Y Acad Sci 1176: 179-190, 2009.
  • Hills M, Lucke K, Chavez EA, Eaves CJ & Lansdorp PM. Probing the mitotic history and developmental stage of hematopoietic cells using single telomere length analysis (STELA).  Blood 113: 5765-5775, 2009.
  • Lansdorp PM. Telomeres and disease.  EMBO J 28: 2532-2540, 2009.
  • Alder JK, Chen JJ, Lancaster L, Danoff S, Su SC, Cogan JD, Vulto I, Xie M, Qi X, Tuder RM, Phillips JAI, Lansdorp PM, Loyd JE & Armanios MY. Short telomeres are a risk factor for idiopathic pulmonary fibrosis.  Proc Natl Acad Sci USA 105: 13051-13056, 2008.
  • Aubert G & Lansdorp P. Telomeres and aging.  Physiol Rev 82: 557-579, 2008.
  • Banuelos CA, Banath JP, MacPhail SH, Zhao J, Eaves CJ, O’Conner MD, Lansdorp PM & Olive PL. Mouse but not human embryonic stem cells are deficient in repair of DNA double-strand breaks induced by ionizing radiation.  DNA Repair 7: 1471-1483, 2008.
  • Berger C, Jensen MC, Lansdorp PM, Gough M, Elliott C & Riddell SR. Adoptive transfer of effector CD8+ T cells derived from central memory cells establishes persistent T cell memory in primates.  J Clin Invest 118: 294-305, 2008.
  • Goldman FD, Aubert G, Klingelhutz AJ, Hills M, Cooper SR, Hamilton WS, Schlueter A, Lambie K, Eaves CJ & Lansdorp PM. Characterization of primitive hematopoietic cells from patients with dyskeratosis congenita.  Blood 111: 4523-4531, 2008.
  • Halaschek-Wiener J, Vulto I, Fornika D, Collins J, Connors JM, Le ND, Lansdorp PM & Brooks-Wilson A. Reduced telomere length variation in healthy oldest old.  Mech Ageing Dev 129: 638-41, 2008.
  • Lansdorp PM. Telomeres, stem cells and hematology.  Blood 111: 1759-1766, 2008.
  • Liu Y, Bohr VA & Lansdorp P. Telomere, telomerase and aging.  Mech Ageing Dev 129: 1-2, 2008.
  • Savage SA, Giri N, Baerlocher GM, Orr N, Lansdorp PM & Alter BP. TINF2, a component of the Shelterin telomere protection complex, is mutated in dyskeratosis congenita.  Am J Hum Genet 82: 501-509, 2008.
  • Tsangaris E, Adams SL, Yoon G, Chitayat D, Lansdorp P, Dokal I & Dror Y. Ataxia and pancytopenia caused by a mutation in TINF2.  Hum Genet 124: 507-13, 2008.
  • Vrisekoop N, van Gent R, de Boer AB, Otto SA, Borleffs JCC, Steingrover R, Prins JM, Kuijpers TW, Wolfs TFW, Geelen SPM, Vulto I, Lansdorp P, Tesselaar K, Borghans JAM & Miedema F. Restoration of the CD4 T cell compartment after long-term highly active antiretroviral therapy without phenotypical signs of accelerated immunological aging.  J Immunol 181: 1573-1581, 2008.
  • Alter BP, Baerlocher GM, Savage SA, Chanock SJ, Weksler BB, Willner JP, Peters JA, Giri N & Lansdorp PM. Very short telomere length by flow fluorescence in situ hybridization identifies patients with dyskeratosis congenita.  Blood 110: 1439-1447, 2007.
  • Armanios MY, Chen JJ-L, Cogan JD, Alder JK, Ingersoll RG, Xie M, Markin C, Lawson WE, Vulto I, Phillips III JA, Lansdorp PM, Greider CW & Loyd JE. Telomerase mutations in families with idiopathic pulmonary fibrosis.  N Engl J Med 356: 1317-1326, 2007.
  • Baerlocher GM, Rice K, Vulto I & Lansdorp PM. Longitudinal data on telomere length in leukocytes from newborn baboons support a marked drop in stem cell turnover around one year of age.  Aging Cell 6: 121-123, 2007.
  • Baerlocher GM, Sloand EM, Young NS & Lansdorp PM. Telomere length in paroxysmal nocturnal hemoglobinuria correlates with clone size.  Exp Hematol Epub ahead of print  2007.
  • Calado RT, Graf SA, Wilkerson KL, Kajigaya S, Ancliff PJ, Dror Y, Chanock SJ, Lansdorp PM & Young NS. Mutations in the SBDS gene in acquired aplastic anemia.  Blood 110: 1141-1146, 2007.
  • Lansdorp PM. Immortal strands? Give me a break.  Cell 129: 1244-1247, 2007.
  • Ouyang Q, Baerlocher G, Vulto I & Lansdorp PM. Telomere length in human natural killer cell subsets.  Ann N Y Acad Sci 1106: 240-252, 2007.
  • Röth A, Dürig J, Himmelreich H, Bug S, Siebert R, Dührsen U, Lansdorp PM & Baerlocher GM. Short telomeres and high telomerase activity in T-cell prolymphocytic leukemia (T-PLL).  Leukemia Epub ahead of print 2007.
  • Sharma S, Stumpo DJ, Balajee AS, Bock CB, Lansdorp PM, Brosh RM, Jr. & Blackshear PJ. RECQL, a member of the RecQ family of DNA helicases, suppresses chromosomal instability.  Mol Cell Biol 27: 1784-1794, 2007.
  • Shepherd BE, Kiem H-P, Lansdorp PM, Dunbar CE, Aubert G, Larochelle A, Seggewiss R, Guttorp P & Abkowitz JL. Hematopoietic stem cell behavior in non-human primates.  Blood 110: 1806-1813, 2007.
  • Westin ER, Chavez E, Lee KM, Gourronc FA, Riley S, Lansdorp PM, Goldman FD & Klingelhutz AJ. Telomere restoration and extension of proliferative lifespan in dyskeratosis congenita fibroblasts.  Aging Cell 6: 383-394, 2007.
  • Xin ZT, Beauchamp A, Calado RT, Bradford JW, Regal JA, Shenoy A, Liang Y, Lansdorp PM, Young NS & Ly H. Functional characterization of natural telomerase mutations found in patients hematological disorders.  Blood 109: 524-532, 2007.
  • Baerlocher GM, Vulto I, de Jong G & Lansdorp PM. Flow cytometry and FISH to measure the average length of telomeres (flow FISH).  Nature Protocols 1: 2365-2376, 2006.
  • Cheung I, Schertzer M, Rose A & Lansdorp PM. High incidence of rapid telomere loss in telomerase-deficient Caenorhabditis elegans.  Nucleic Acids Res 34: 96-103, 2006.
  • Lansdorp PM. Stress, social rank and leukocyte telomere length.  Aging Cell 5: 583-584, 2006.
  • Savage SA, Stewart BJ, Weksler BB, Baerlocher GM, Lansdorp PM, Chanock SJ & Alter BP. Mutations in the reverse transcriptase component of telomerase (TERT) in patients with bone marrow failure.  Blood Cells, Molecules and Diseases 37: 134-136, 2006.